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S Lee, KH Park, JH Kang, YJ Kim, JS Seo; The Effect of Heat Shock Protein on Retinal Ganglion Cell Damage after Partial Lesion of the Optic Nerve in Mice . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2197.
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Purpose:Heat shock protien (Hsp) 70 has been known to have neuroprotective effect on retinal ganglion cells(RGCs) in vitro and in a rat glaucoma model. This study was to determine the effect of Hsp70 on the RGCs in a partial crush injury model of the optic nerve by comparison between hsp 70.1 knockout mice and of wild type mice. Methods:The optic nerve of the hsp 70.1 knockout mice and of wild type C57BL/6 mice were subjected to partial crush injury using aneurysmal clip(110G). Loss of RGCs was monitored as a percentage of cells remaining relative to the contralateral sham-operated eye. Immunohistochemical staining for Hsp 70 was performed. The number of neurons remaining in the ganglion cell layer (GCL) after 3 weeks was compared between the hsp 70.1 knockout mice and the wild type mice. Results:Remaining cells in the GCL was 69.0% ± 0.1% (mean ± SD) in the hsp 70.1 knock-out mice and 67.4% ± 0.1% in the wild type mice without any significant difference between the two groups (n=8, Mann-Whitney test). Immonohistochemical staining showed Hsp 70 induction in the crushed region of the optic nerve but minimal induction in the RGCs to show any significant difference between two groups. Conclusion:Hsp 70 was not induced enough in RGCs to protect RGCs in the partial crush injury model of the optic nerve.
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