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M Moschos, S Eperon, Y Guex-Crosier; Up-regulation of Eotaxin in Tears of Patients Wearing Contact Lenses . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2210.
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Purpose: Giant-papillary conjunctivitis occurs because of allergy and/or intolerance to contact lenses, their cleaning products or preservatives. Increase of eotaxin has been found in tears of severe allergic patients. It is a recently discovered C-C chemokine with potent and selective chemotactic effect for eosinophils. The purpose of this study is the quantitative determination of eotaxin in tears of patients wearing contact lenses and its comparison with normal subjects. Methods: Around 10 µl of tears were collected with glass capillaries in 8 patients (2 male, 6 female) wearing contact lenses and in 10 (1 male, 9 female) normal subjects. Tears were immediately frozen. Approximate estimation of both deposits in the surface of the contact lenses (grade 1-4) and papillae in the superior tarsal conjunctiva (grade 1-4) was performed. Tears were processed by ELISA using a purified mouse anti-human eotaxin monoclonal antibody as capture antibody and a biotinylated mouse anti-human eotaxin monoclonal antibody as detecting antibody (Becton Dickinson-Pharmingen). For the statistical analysis of the results the Wilcoxon/ Kruskal-Wallis test was used. Results: The mean concentration of eotaxin was found 2446 774 (SEM) pg/ml (range 1330-3968 pg/ml) in patients wearing contact lenses and 1291 323 g/ml (range 611-1801 pg/ml) in normal subjects. The difference was statistically significant (p<0.005). Conclusion: In this preliminary study, we successfully detected high concentrations of eotaxin in tear samples from patients wearing contact lenses. Therefore, the amount of eotaxin in these patients may play an important role and may be used to determine their tendency to allergic ocular diseases.
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