Purchase this article with an account.
M Kamiryo, Y Takahashi, H Yamashita; Immunohistological Observation of Amniotic Membrane Transplantation in Ocular Surface Diseases . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2240.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: In the treatment of the refractory ocular surface diseases, amniotic membrane transplantation(AMT) is useful to control scar formation. To clarify the mechanisms of amniotic membrane transplantation affecting scar formation, we observed the wound healing process in the clinical samples immunohistologically. Method: The specimens(the corneal and conjunctival tissure) were obtained from the patient with pterygium(2 cases), chemical burn(1 case), and corneal perforation after fungus infection(1 case). In the case with corneal perforation, AMT was performed to treat the tissue defect. These after the corneal transplantation was performed, and the excised specimens containing the amniotic membrane tissue was obtained. We observed the histological stutus of the transplanted amniotic membrane, and compared the expression of laminin, the type IV collagen, ICAM-1 and LFA-1 immunohistochemically between the cases with and without AMT. Results: In the case with AMT, early reepithelization was observed on amniotic membrane, but extracellular matrix deposition was not clear. Immunohistologicaiiy, the expression levels of laminin were similar between the cases with and without AMT in epitherial cells. The expression levels of type IV collagen were different:Type IV collagen was detected around the vascular endotherial cells in the specimens from the cases without AMT, however, was not detected around that in the case with AMT. Conclusion: These results support that AMT accelerate early reepithelization, without extracellular matrix deposition.
This PDF is available to Subscribers Only