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H Yokoi, JW Streilein; Regulator T Cells Generated In Vitro by TGFß-Treated Antigen Presenting Cells Endow Fresh Antigen Presenting Cells with ACAID-Inducing Capabilities . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2274.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To determine whether regulatory CD4 T cells activated in vitro by antigen-pulsed, transforming growth factor-ß (TGF-ß)-treated antigen presenting cells (APCs) alter the ability of fresh APCs to activate naive T cells in vitro. Methods:Regulator T cells, generated by stimulating T cells from DO11.10 Tcr transgenic mice with OVA-pulsed, TGF-ß2-treated APCs, and control DO11.10 T cells were incubated with fresh, OVA-pulsed APCs for 24 hr. These cultures were separated into supernatants (SN) and adherent cells. The latter were used (a) to stimulate naïve DO11.10 T cells in vitro, and (b) to injected i.v. into naïve BALB/c mice that were immunized subsequently with OVA in CFA. SN was added to cultures containing naïve DO11.10 T cells plus OVA-pulsed APCs. IFN-γ and IL-4 production in vitro were assayed by ELISA. Results:OVA-pulsed APCs exposed to regulator T cells (a) failed to induce naïve DO11.10 T cells to secrete IFN-γ in vitro, and (b) prevented BALB/c mice from acquiring OVA-specific DH. Supernatants from cultures containing regulator T cells and OVA-pulsed APCs suppressed IFN-γ production when added to DO11.10 T cells stimulated by OVA-pulsed APCs. Conclusion:Regulatory CD4 T cells, generated in vitro by exposure to OVA-pulsed, TGF-ß2-treated APCs, endow fresh APC with ACAID-inducing properties. Soluble factor(s) released from regulator T cells are at least partly responsible for this direct effect on APCs.
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