December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The effect of IFN-alpha, IFN-gamma, and TGF-beta on retinal pigment epithelium induced apoptosis in Jurkat T-cells
Author Affiliations & Notes
  • KA Rezai
    Kresge Eye Institute Wayne State University Detroit MI
  • L Farrokh-Siar
    Department of Ophthalmology & Visual Science University of Chicago Chicago IL
  • EM Gasyna
    Department of Ophthalmology & Visual Science University of Chicago Chicago IL
  • SC Patel
    Department of Ophthalmology & Visual Science University of Chicago Chicago IL
  • G van Seventer
    Boston University School of Health Department of Environmental Health Boston MA
  • JT Ernest
    Department of Ophthalmology & Visual Science University of Chicago Chicago IL
  • Footnotes
    Commercial Relationships   K.A. Rezai, None; L. Farrokh-Siar, None; E.M. Gasyna, None; S.C. Patel, None; G. van Seventer, None; J.T. Ernest, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2287. doi:
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    • Get Citation

      KA Rezai, L Farrokh-Siar, EM Gasyna, SC Patel, G van Seventer, JT Ernest; The effect of IFN-alpha, IFN-gamma, and TGF-beta on retinal pigment epithelium induced apoptosis in Jurkat T-cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2287.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To examine the up-regulatory effect of different cytokines on retinal pigment epithelium induced apoptosis in human Jurkat T-cells (Jkt). Methods:Pure cultures of human fetal retinal pigment epithelial cells (HFRPE) were isolated and cultured. HFRPE cells were incubated with IFN-a, IFN-g, TGF-b, or IFN-g and TGF-b simultaneously for 72 hours. Then the activated HFRPE cells were co-cultured with Jkt cells for 48 hours. Apoptosis of Jkt cells was determined with Annexin V staining and flowcytometry. Results:Both IFN-a and IFN-g activated HFRPE cells induced an increased amount of apoptosis in Jkt cells (20.51+2.4 % and 34.47+2.01 %) respectively. TGF-b activated HFRPE cells, however, did not induce a significant amount of apoptosis in Jkt cells (14.76+1.33 %) in comparison to non-activated HFRPE cells (15.68+2.31). The apopstosis rate in Jkt cells after incubation with both IFN-g and TGF-b activated HFRPE cells was similar to only IFN-g activated HFRPE cells (37.09+3.5 %). Conclusion:These results indicate that type I and type II IFNs can similarly up-regulated the HFRPE induced apoptosis in Jkt T-cells. TGF-b, however, could not up-regulate the HFRPE induced apoptosis in Jkt cells. These findings may help us to better understand the modulatory effect of pro- and anti-inflammatory cytokines on immune suppressive characteristics of HFRPE cells.

Keywords: 433 immune tolerance/privilege • 567 retinal pigment epithelium • 323 apoptosis/cell death 
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