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K Hosseini, WF March, RJ Erckens, FH M Jongsma, F Hendrikse, M Motamedi; Noninvasive Assessment of Local Ceftazidime and Amphotericin B Concentration in Ocular Tissue by Raman Spectroscopy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2310.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To establish a noninvasive means for effective monitoring of the local concentrations of Ceftazidime and Amphotericin B in the ocular media. Methods:An integrated CCD-based Raman spectroscopic system and argon laser, engineered specifically for ophthalmic application, were employed to noninvasively assess the pharmacokentics of Ceftazidime and Amphotericin B present in the anterior chamber of rabbit eye following local delivery of drug. Results:The unique spectral characteristics exhibited by each drug were identified and discerned from the spectral features of ocular tissue. The spectral features of Ceftazidime appeared near 1028, 1506, 1586 (the strongest peak) and 1641 wavenumbers. Amphotericine B exhibited its spectral features in the region of 1156.5 and 1556 wavenumbers. The amplitudes of both drugs’ spectral peaks were linearly dependent upon their local concentration within the anterior chamber. Conclusion:The results of our study strongly indicate that Raman spectroscopy can serve as an effective, noninvasive means of evaluating Ceftazidime and Amphotericin B concentrations within the ocular media. The application of Raman spectroscopy could permit the physician to determine the presence and concentration of the investigated drug, thereby allowing local drug concentration to remain within therapeutic levels within the eye.
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