December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effects of Growth Factors on a Smooth Muscle Actin Expression in Porcine Lens Epithelial Cells
Author Affiliations & Notes
  • Y Hesse
    Dept of Ophthalmology University of Ulm Ulm Germany
  • GK Lang
    Ulm Germany
  • J Kampmeier
    Ulm Germany
  • GE Lang
    Ulm Germany
  • Footnotes
    Commercial Relationships   Y. Hesse, None; G.K. Lang, None; J. Kampmeier, None; G.E. Lang, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2337. doi:
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      Y Hesse, GK Lang, J Kampmeier, GE Lang; Effects of Growth Factors on a Smooth Muscle Actin Expression in Porcine Lens Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2337.

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Abstract

Abstract: : Purpose: To determine whether basic fibroblast growth factor (bFGF), transforming growth factor-beta 2 (TGF-b 2), epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) can influence transdifferentiation from lens epithelial cells to myofibroblasts in cultured porcine lens epithelial cells (LECs). The transdifferentiation process was monitored by a-smooth muscle actin (a-SMA) expression. Methods: Porcine LECs were cultured for 7 days in serum-free medium with or without 1 to 50 ng/ml bFGF, TGF-b 2, EGF or IGF-I and in medium containing 10% fetal bovine serum. Alpha-SMA was detected immunocytochemically with a mouse monoclonal antibody, and the relative numbers of a-SMA-positive cells, the P/T ratio, were calculated. Results: The P/T ratio of the LECs cultured for 7 days in serum-free medium was 36% ± 11.9 (mean ± SD). BFGF significantly reduced the ratio in a dose dependent manner from 42.9% ± 4.1 to 11.2% ± 7.3 at a concentration of 50ng/ml (p<0.0001). EGF reduced the P/T ratio significantly (p=0.05) from 39.8% ± 14.8 to 25.1% ± 15.7 when 50ng/ml were applied. IGF-1 (10ng/ml) reduced the P/T-ratio from 27.8% ± 15.4 to 16.8% ± 5.8, but the reduction was not statistically significant (p=0.0787). TGF-b 2 (50ng/ml) slightly increased the relative number of a-SMA-positive cells from 33.8% ± 13.2 to 44.2% ± 13.8. However, this increase was not statistically significant (p=0.1202) during a culture period of 7 days. Cells cultured in serum-supplemented medium presented an overall P/T ratio of 11% ± 7.4 Conclusion: BFGF and EGF significantly reduced the a-SMA expression of LECs indicating a protective effect concerning transdifferentiation from LECs to myofibroblasts. TGF-b 2 and IGF-1 had no significant effect during a culture period of 7 days.

Keywords: 423 growth factors/growth factor receptors • 522 posterior capsular opacification (PCO) 
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