December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Sequence Conservation of Lens Epithelium-Derived Growth Factor (LEDGF)
Author Affiliations & Notes
  • N Adachi
    Center for Ophthalmic Research Brigham and Women's Hospital Department of Ophthalmology Harvard Medical School Boston MA
  • DP Singh
    Boston MA
  • T Shinohara
    Boston MA
  • LT Chylack
    Boston MA
  • Footnotes
    Commercial Relationships   N. Adachi, None; D.P. Singh , None; T. Shinohara , None; L.T. Chylack, None. Grant Identification: NIH grants EY1058 and EY10824, Mass Lions Eye Res. Fund, and Foundation for Fighting Blindness
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2341. doi:
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    • Get Citation

      N Adachi, DP Singh, T Shinohara, LT Chylack; Sequence Conservation of Lens Epithelium-Derived Growth Factor (LEDGF) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2341.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: LEDGF enhances survival of many cells by binding to heat shock (HSE) and stress-related (STRE) elements to activate stress-related genes. To gain insight into LEDGF’s structure, evolutionary conservation, and ecliptic functional role, we determined a bovine LEDGF cDNA sequence. We also obtained LEDGF sequences of mouse, rat, chicken, pig, and fruit fly from Database and assessed their sequence homologies. Methods: A bovine brain cDNA library was screened with a [32P]-labeled human LEDGF cDNA probe. The cDNA clone was isolated, its DNA sequence was determined, and the polypeptide sequence was deduced. The LEDGF homologues of other species were obtained from the GenBank database using the BLAST program. For domain/motif structure analysis of the protein, the InterProScan and PSORT II analysis were used. Size of LEDGF mRNAs was determined by Northern blot analyses. Results: The entire bovine LEDGF polypeptide sequence was determined. The whole LEDGF sequences of human, bovine, and mouse were compared; ≷95% of the polypeptide sequence was conserved among them. LEDGF sequences contained several functional domains (e.g. seven nuclear localization signals (NLS), six DNA binding domains (three helix-turn-helixes, a coiled-coil, a basic leucine zipper, and an A-T hook), Tat-, PWWP-, and a TFS2N-domains). Among vertebrates we have studied, sequence conservation in each domain was extremely high. Size of mRNAs of LEDGF was found to be 3.4 kb. Conclusion: The high sequence conservations of these domains (NLS, Tat, a basic leucine zipper, and a helix-turn-helixes) are likely important for cell survival. .

Keywords: 417 gene/expression • 476 molecular biology • 423 growth factors/growth factor receptors 

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