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J Lee, T Yoon, B Oum; Toxicity of Indocyanine Green Injected into Subretinal Space Experimentally . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2472.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To facilitate peeling of internal limiting membrane in vitrectomy for macular hole, indocyanine green(ICG) is used to stain internal limiting membrane. During procedure, subretinal tissue can be contacted with ICG through macular hole. We evaluated toxicity of indocyanine green to subretinal tissue. Methods: BSS and ICG of various concentration (0.06%, 0.25%, and 0.5%) were injected into subretinal space of rabbit after vitrectomy. Injected BSS and ICG were removed after 1 minute. After 3days, 1 week, and 4 weeks, the eyes were enucleated and evaluated with electron microscopy and light microscopy. Results: When ICG solutions of 0.25% or higher concentration were injected into subretinal space, degenerative changes of photorecpter were found only by electron microscopy after 3 days and outer nuclear layer and photorecepter layer showed significant destructive change by light and electon microscopy after 1 week. When BSS or 0.06% ICG solution were injected, no significant change were found with light or electron microscopy at any time. Conclusion: The results of this study suggest that ICG of 0.25% or higher concentration has toxicity to subretinal tissue and may cause adverse effect on functional outcome of macular hole surgery.
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