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X Ou; The Immunoactivity of Retinal S-Antigen in Retinoblastoma . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2585.
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Purpose:To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations. Methods:Twenty-three cases of retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody, MabA9C6, which defines an epitope in S-antigen retained in fixed-tissue sections, was used to study S-antigen expression in 23 cases of retinoblastomas. S-antigen was localized by the biotinavidin indirect immunoperoxidase technique and purified MabA9C6 ascites fluid was used with 1:100. The whole proceedure could be finished within a few hours. Results:The S-antigen immunoactivity was observed in different patterns: the "normal" photoreceptor elements incorporated in 5 cases of growing tumors; 5 of 7 Fleurettes and E-W rosettes; and scattered tumor cells in 58% of the cases. Conclusion:Our result suggests that the expression of S-antigen in retinoblastoma may be used to assess the degree of tumor differentiation as another tumor marker in retinoblastoma.
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