December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The Effect Of Amyloride And Procaine On Expression Of Heat Shock Protein 70 In Rat Retina
Author Affiliations & Notes
  • M Tunc
    AIBU-Duzce Medical School Duzce Turkey
    Dept of Ophthalmology
  • A Güven
    Histology Dept AIBU Duzce Medical School Duzce Turkey
  • M Cam
    AIBU-Duzce Medical School Duzce Turkey
    Histology Dept
  • M Kaya
    AIBU-Duzce Medical School Duzce Turkey
    Dept of Ophthalmology
  • G Aktan
    Deptof Ophthalmology
    AIBU-Duzce Medical School Duzce Turkey
  • I Ziyal
    Dept of Neurosurgery
    AIBU-Duzce Medical School Duzce Turkey
  • Footnotes
    Commercial Relationships   M. Tunc, None; A. Güven, None; M. Cam, None; M. Kaya, None; G. Aktan, None; I. Ziyal, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2730. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M Tunc, A Güven, M Cam, M Kaya, G Aktan, I Ziyal; The Effect Of Amyloride And Procaine On Expression Of Heat Shock Protein 70 In Rat Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2730.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To identify the effects of amyloride and procaine in retinal expression of heat shock protein 70 (HSP 70) following traumatic injury. Methods: We induced traumatic stress by performing experimental spinal injury in 36 male Wistar-albino rats. We randomised the animals into three groups. Group I received no medications, Group II received intraperitoneal (IP) Procaine 15mg/kg, Group III received Amyloride 30 mg/kg IP. We sacrificed half of the animals in each groups at 12th hour and the remaining half in 24 hours. Following immidiate enucleation we isolated the retinae and detected HSP 70 immunoreactivity in each group. Results: There was moderate expression of HSP 70 in the outer nuclear and inner plexiform layer of the retinae after 12 hours and marked staining with HSP 70 antibodies 24 hours after the injury in all subjects which did not receive any medication. There was little or no immunoreaction to HSP 70 in rat retinae which received procaine or amyloride. Conclusion: Decreased expression of HSP 70 in the retina by procaine and amyloride indicates their neuroprotective role following traumatic stress.

Keywords: 592 stress response • 608 trauma • 489 neuroprotection 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×