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RF Gariano, MR Sierra-Honigmann, M Friedlander; Localization Leptin Receptors During Retinal Vascular Development . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2760.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Leptin, a pleiotropic cytokine with angiogenic activity, is implicated in retinal diseases with fibrovascular proliferation. To determine if leptin may relate to retinal vascular development, we examined the temporal and spatial appearance of leptin receptor in mouse retina during the period of retinal developmental vascularization. Methods: Immunohistochemistry was performed on whole-mounted retinae from mice ranging in age from post-natal day 1 to adult. Antisera were used to designate vessels (collagen IV and griffonia simplicifolia isolectin), astrocytes (GFAP), leptin and leptin receptor. Results: At all ages examined, leptin receptor was predominantly localized to astrocyte cell bodies and processes in the nerve fiber layer. Leptin receptor in astrocytes appeared only in regions already possessing blood vessels; peripheral to growing vessels, astrocytes were GFAP-positive and leptin receptor-negative. After age 10 days, leptin receptor was seen in all astrocyte cell bodies and processes surrounding vessels. Lower levels of leptin receptor immunoreactivity were also seen in blood vessels, more so on veins than arteries, and in vertically-oriented processes presumed to be Muller cells. The retinal vascular morphology in murine strains defective in leptin and leptin receptor synthesis (ob/ob and db/db, respectively) appeared normal at age 7 weeks, though at age 5 weeks the degree of vessel retraction (a form of remodeling) was greater in db/db than in ob/ob and wild-type retinae. Conclusion: Leptin receptor is present in retinal glia during the period of vascular development, and appears to a lesser degree in vascular elements. Absence of the functional leptin receptor exerts only subtle effects on retinal vessel formation. These results may be of interest in light of leptin's actions in angiogenesis, fibrosis, and vascular permeability.
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