December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Somatostatin Subtype Receptor 4 Expression in Mouse and Rat Retina
Author Affiliations & Notes
  • N Brecha
    Depts of Neurobiology & Medicine Jules Stein Eye Institute UCLA School of Medicine VAGLAHS Los Angeles CA
  • A Vila
    Depts of Neurobiology & Medicine Jules Stein Eye Institute UCLA School of Medicine VAGLAHS Los Angeles CA
  • J Allen
    Laboratory of Cognitive and Developmental Neuroscience The Babraham Institute Babraham United Kingdom
  • Footnotes
    Commercial Relationships   N. Brecha, None; A. Vila, None; J. Allen, None. Grant Identification: NEI Grant EY04067 and VA Career Scientist Award
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2768. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      N Brecha, A Vila, J Allen; Somatostatin Subtype Receptor 4 Expression in Mouse and Rat Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2768.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Somatostatin (SRIF) in the rat and mouse retina is expressed by sparsely occurring amacrine and displaced amacrine cells. Their processes are mainly distributed to laminae 1 and 5 of the inner plexiform layer (IPL). SRIF influences neuronal activity by interacting with five somatostatin receptors, which are G-protein-coupled receptors. We characterized the expression and cellular localization of the somatostatin subtype receptor 4 (sst4) in mouse and rat retina. Methods: Rat and mouse retinas were fixed with 4% paraformaldehyde and processed for immunohistochemistry using an affinity-purified antibody to the C-terminus of sst4. Antibody specificity was evaluated by immunostaining with the sst4 antibody preabsorbed with 10-6M C-terminus sst4 peptide fragment and by immunostaining of retinas from mice with a homozygous deletion of the sst4 gene. Results: Specific sst4 immunoreactivity was localized to numerous multistratified processes in all regions of the IPL and processes in the axon layer and to medium and large cell bodies in the ganglion cell layer. Immunoreactivity was localized to the plasma membrane and cytoplasm. Immunostaining was not observed in the optic nerve or central nervous system nuclei that receive retinal ganglion cell projections. Immunostaining was not observed in sections incubated with sst4 antibodies preabsorbed with sst4 C-terminus peptide and in homozygous mice lacking sst4. Conclusion: These findings show that the sst4 is mainly localized to the dendrosomatic compartment of multistratified ganglion cells. These observations are consistent with an earlier report of sst4 mRNA in cells located in the ganglion cell layer. The difference in the distribution of SRIF- and sst4-immunoreactive processes supports the hypothesis that this peptide acts in a paracrine manner to influence multiple cell types distributed throughout the retina.

Keywords: 488 neuropeptides • 415 ganglion cells • 434 immunohistochemistry 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×