December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Adenosine Attenuates the Effect of ATP P2X7 Agonist on Retinal Ganglion Cells
Author Affiliations & Notes
  • X Zhang
    Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • C Friedman
    Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • AM Laties
    Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • CH Mitchell
    Physiology University of Pennsylvania Philadelphia PA
  • Footnotes
    Commercial Relationships   X. Zhang, None; C. Friedman, None; A.M. Laties, None; C.H. Mitchell, None. Grant Identification: NIH EY10009-09, Jody Sack Fund, The Foundation Fighting Blindness, Research to Prevent Blindness
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2777. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      X Zhang, C Friedman, AM Laties, CH Mitchell; Adenosine Attenuates the Effect of ATP P2X7 Agonist on Retinal Ganglion Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2777.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To investigate the effect of ATP agonists and adenosine on intracellular Ca2+ levels of rat retinal ganglion cells (RGCs). Methods: Retinae were dissociated from Long Evan rat pups and cultured overnight. Cells were loaded with the dye fura-2 and Ca2+ levels in RGCs were measured ratiometrically using a fluorescent imaging system. Results: The Ca2+ of RGCs was elevated by a variety of purines; at 10µM, the efficacy ranking was BzATP≷UTP≷ATP. While Ca2+ returned to baseline after removal of ATP levels remained elevated after BzATP removal. Adenosine itself did not raise Ca2+ levels, but greatly attenuated the BzATP effect. When present ∼2 min before and during the BzATP application, adenosine reduced the peak Ca2+ significantly and the sustained elevation in Ca2+ concentration following BzATP was eliminated. Conclusion: The Ca2+ levels of rat RGCs can be elevated by ATP, UTP and BzATP but not by adenosine. The sustained elevation of Ca2+ after BzATP suggests that stimulation of the P2X7 receptor could damage cells. Adenosine may reduce this damage.

Keywords: 306 adenosine • 415 ganglion cells • 541 receptors: pharmacology/physiology 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×