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Y Kishikawa, H Gong, T Kitaoka, T Amemiya, K Takaya, M Tozu, Y Ohashi; Tof-sims Analysis Of Elements And Organic Substances In Proliferative Tissues Of Age Related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2797.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: The possibility that a deficiency of some trace element causes macular diseases has been suggested. The purpose of the present study is to examine the elements and organic substances in the proliferative tissues in age related macular degeneration (ARMD), with a Time Of Flight Secondary Ion Mass Spectrometer (TOF-SIMS). Methods: Choroidal new capillaries were excised at vitreous surgery from five patients with ARMD. The specimens were washed in distilled water, because intraocular irrigating solution includes elements, placed on silicon wafer plates, and stocked in vials, which were put into liquid nitrogen and preserved. For TOF-SIMS examination the specimens were dried, and elements ( Na, Mg, K, Ca, Al, Fe, Mn, Zn, Cu ), vitamins ( vitamin A, vitamin E ) and fatty acids ( palmitic acid, oleic acid, stearic acid) were measured with TOF-SIMS. Certain areas of the surface of each specimen received primary ion beam sputtering for 5 minutes, and counts of each substance were recorded. Two sites in each specimen were examined, and the average count of each substance was calculated. Results: Na showed the highest count, but we may have to take into consideration the fact that intraocular irrigating solution contains much Na, and TOF-SIMS is especially sensitive to Na. Of the trace elements Fe had the highest count. It came perhaps from the hemoglobin of red blood cells. Mn had the second highest count, Cu the third highest and Zn the lowest count. The proliferative tissue contained more vitamin A than vitamin E. Of the fatty acids oleic acid had the highest count, and palmitic acid had the lowest. Conclusion: TOF-SIMS could easily show elements and organic substances such as vitamins and fatty acids in the choroidal new capillaries of patients with ARMD. These data must be compared with those found in various vitreoretinal proliferative diseases.
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