December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Tof-sims Analysis Of Elements And Organic Substances In Proliferative Tissues Of Age Related Macular Degeneration
Author Affiliations & Notes
  • Y Kishikawa
    Ophthalmology and Visual Science Nagasaki Univ Sch Med Nagasaki Japan
  • H Gong
    Department of Ophthalmology and Visual Sciences Nagasaki University School of Medicine Nagasaki Japan
  • T Kitaoka
    Department of Ophthalmology and Visual Sciences Nagasaki University School of Medicine Nagasaki Japan
  • T Amemiya
    Department of Ophthalmology and Visual Sciences Nagasaki University School of Medicine Nagasaki Japan
  • K Takaya
    Department of Anatomy 2 Toyama Medical and Pharmaceutical University Faculty of Medicine Toyama Japan
  • M Tozu
    Ulvac-phi Incorporated Chigasaki Japan
  • Y Ohashi
    Ulvac-phi Incorporated Chigasaki Japan
  • Footnotes
    Commercial Relationships   Y. Kishikawa, None; H. Gong, None; T. Kitaoka, None; T. Amemiya, None; K. Takaya, None; M. Tozu, None; Y. Ohashi, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2797. doi:
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    • Get Citation

      Y Kishikawa, H Gong, T Kitaoka, T Amemiya, K Takaya, M Tozu, Y Ohashi; Tof-sims Analysis Of Elements And Organic Substances In Proliferative Tissues Of Age Related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2797.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: The possibility that a deficiency of some trace element causes macular diseases has been suggested. The purpose of the present study is to examine the elements and organic substances in the proliferative tissues in age related macular degeneration (ARMD), with a Time Of Flight Secondary Ion Mass Spectrometer (TOF-SIMS). Methods: Choroidal new capillaries were excised at vitreous surgery from five patients with ARMD. The specimens were washed in distilled water, because intraocular irrigating solution includes elements, placed on silicon wafer plates, and stocked in vials, which were put into liquid nitrogen and preserved. For TOF-SIMS examination the specimens were dried, and elements ( Na, Mg, K, Ca, Al, Fe, Mn, Zn, Cu ), vitamins ( vitamin A, vitamin E ) and fatty acids ( palmitic acid, oleic acid, stearic acid) were measured with TOF-SIMS. Certain areas of the surface of each specimen received primary ion beam sputtering for 5 minutes, and counts of each substance were recorded. Two sites in each specimen were examined, and the average count of each substance was calculated. Results: Na showed the highest count, but we may have to take into consideration the fact that intraocular irrigating solution contains much Na, and TOF-SIMS is especially sensitive to Na. Of the trace elements Fe had the highest count. It came perhaps from the hemoglobin of red blood cells. Mn had the second highest count, Cu the third highest and Zn the lowest count. The proliferative tissue contained more vitamin A than vitamin E. Of the fatty acids oleic acid had the highest count, and palmitic acid had the lowest. Conclusion: TOF-SIMS could easily show elements and organic substances such as vitamins and fatty acids in the choroidal new capillaries of patients with ARMD. These data must be compared with those found in various vitreoretinal proliferative diseases.

Keywords: 308 age-related macular degeneration • 554 retina • 508 pathology techniques 
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