December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
A Novel Method of Imaging the Rat Optic Nerve Head In Vivo With En-face Optical Coherence Tomography (OCT): Correlation With Confocal Microscopy
Author Affiliations & Notes
  • MF Cordeiro
    Moorfields Eye Hospital and Institute of Ophthalmology London United Kingdom
    Department of Pathology Departments of Glaucoma and Pathology
  • L Guo
    Department of Pathology Departments of Glaucoma and Pathology
    Institute of Ophthalmology London United Kingdom
  • T Morrissey
    Department of Pathology Department of Visual Science
    Institute of Ophthalmology London United Kingdom
  • CS Sethi
    Department of Pathology Department of Visual Science
    Moorfields Eye Hospital and Institute of Ophthalmology London United Kingdom
  • PM Munro
    Department of Pathology Departments of Glaucoma and Pathology
    Institute of Ophthalmology London United Kingdom
  • DF Garway Heath
    Glaucoma Research Unit
    Moorfields Eye Hospital and Institute of Ophthalmology London United Kingdom
  • FW Fitzke
    Department of Pathology Department of Visual Science
    Institute of Ophthalmology London United Kingdom
  • Footnotes
    Commercial Relationships   M.F. Cordeiro, None; L. Guo, None; T. Morrissey, None; C.S. Sethi, None; P.M. Munro, None; D.F. Garway Heath, None; F.W. Fitzke, None. Grant Identification: Wellcome Trust, LORS
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2883. doi:
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      MF Cordeiro, L Guo, T Morrissey, CS Sethi, PM Munro, DF Garway Heath, FW Fitzke; A Novel Method of Imaging the Rat Optic Nerve Head In Vivo With En-face Optical Coherence Tomography (OCT): Correlation With Confocal Microscopy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2883.

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Abstract

Abstract: : Purpose: En-face optical coherence tomography (OCT) is a new high-resolution technique that can be used to image the optic nerve head (ONH). As the rat model is increasingly recognised as a useful tool for studying glaucoma, we have validated the results of en-face OCT imaging of rat ONH in vivo with confocal microscopy (CM) of the same eyes analysed post-mortem. Methods: 10 Brown Norway underwent general anaesthesia during which en-face OCT was used to image the fundus of each eye. Animals were killed soon after and enucleated eyes were fixed in paraformaldehyde and labeled with antibodies and special stains for axons, ganglion cell processes and extracellular matrix. 3D reconstructions of each rat ONH with CM were then compared to those obtained in vivo with en-face OCT. Results: Tomographic images of the ONH in each rat eye were reconstructed from multiple en-face OCT optical sections, as previously described. The images obtained were of high resolution showing clear detailed features of depth and topography. The mean posterior scleral thickness was OCT = 89 ± 10 microns, CM = 78 ± 12 microns. The z-profile of structures observed with en-face OCT were similar to those obtained with CM. Optical sections generated by OCT were 5 microns thick each, compared to CF which were 6.25 microns. Depth analysis of the different tissue planes and surface topography confirmed a similar relationship between the two methods of 3D reconstruction. Conclusion: The use of OCT tomographic imaging of the rat ONH in vivo to assess anatomical features of the ONH has been validated by our histological findings with CM. The difference in scleral thickness measurement may be due to tissue shrinkage post-mortem or differences in defining tissue borders with the two techniques. Obviously, en-face OCT is an advantageous method of imaging as it allows repeated in vivo measurements. Our results suggest that the high resolution of the OCT makes it a very useful tool for objectively measuring damage in ONH disease. More importantly, en-face OCT represents an exciting advance for monitoring changes in vivo at the ONH, particularly in longitudinal studies of the rat model of glaucoma. Acknowledgements: Adian Gh. Podoleanu, David A. Jackson.

Keywords: 498 optic disc • 432 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 316 animal model 
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