December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
AAV-Mediated Retinal Transfer Of Anti-angiogenic Genes In A Murine Model Of Retinopathy Of Prematurity (ROP)
Author Affiliations & Notes
  • KC Behling
    Dept of Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • A Auricchio
    Dept of Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • EE O'Connor
    Dept of Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • AM Maguire
    Dept of Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • MJ Tolentino
    Dept of Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • JM Wilson
    Institute for Human Gene Therapy University of Pennsylvania Philadelphia PA
  • J Bennett
    Dept of Ophthalmology Scheie Eye Institute University of Pennsylvania Philadelphia PA
  • Footnotes
    Commercial Relationships   K.C. Behling, None; A. Auricchio, None; E.E. O'Connor, None; A.M. Maguire, None; M.J. Tolentino, None; J.M. Wilson, Targeted Genetics I, C, P; GlaxoSmithKline F, P; J. Bennett, None. Grant Identification: Support:EY10820,EY12156,EY13140,5T32EY07035,JDF,FFB,Steinbach,Mackall and F.M. Kirby Founds.,RPB
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2894. doi:
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    • Get Citation

      KC Behling, A Auricchio, EE O'Connor, AM Maguire, MJ Tolentino, JM Wilson, J Bennett; AAV-Mediated Retinal Transfer Of Anti-angiogenic Genes In A Murine Model Of Retinopathy Of Prematurity (ROP) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2894.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Retinal neovascularization is a significant cause of morbidity and blindness. Anti-angiogenic factors have been shown to inhibit neovascularization and therefore may provide important therapies for ocular neovascular processes. We hypothesize that viral delivery of anti-angiogenic genes to retinal cells can result in inhibition of retinal neovascularization. Methods:Postnatal day (P) 3-5 C57Bl/6 mice were injected subretinally in one eye with one of the following adeno-associated viruses (AAV): AAV-PEDF, AAV-endostatin, or AAV-TIMP-3. Contralateral eyes were injected with a control virus containing the same promoter (CMV). From P7-P12, the mice were placed in a 75% oxygen environment after which time they were reared in room air [see IOVS 1994;35:101-11]. At P17-19 the extent of abnormal blood vessel growth was evaluated by angiography and histopathology. Transgene expression analyses confirmed expression of the relevant transgenes. Results:There was decreased neovascular pathology in eyes treated with each of the experimental vectors but not with the control vector. Conclusion:AAV-mediated delivery of any of a variety of anti-angiogenic genes can result in decreased neovascularization in a mouse model of ROP. This suggests that similar strategies may result in decreased retinal neovascularization in other neovascular pathologies of the eye such as diabetic retinopathy and choroidal neovascularization.

Keywords: 419 gene transfer/gene therapy • 572 retinopathy of prematurity • 483 neovascularization 
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