December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Role of Epithelium in Myofibroblast Transformation in the Cornea
Author Affiliations & Notes
  • SE Wilson
    Department of Ophthalmology Univ of Washington Sch of Med Seattle WA
  • RR Mohan
    Department of Ophthalmology Univ of Washington Sch of Med Seattle WA
  • JW Hong
    The Department of Ophthalmology Korea University Seoul Democratic People's Republic of Korea
  • RR Mohan
    Department of Ophthalmology Univ of Washington Sch of Med Seattle WA
  • JS Lee
    Department of Ophthalmology Research Institute of Medical Science College of Medicine Pusan National University Pusan Democratic People's Republic of Korea
  • R Ambrósio
    Department of Ophthalmology Univ of Washington Sch of Med Seattle WA
  • AE K Hutcheon
    Schepen's Eye Research Institute Harvard University Boston MA
  • J Zieske
    Schepen's Eye Research Institute Harvard University Boston MA
  • Footnotes
    Commercial Relationships   S.E. Wilson, None; R.R. Mohan, None; J.W. Hong, None; R.R. Mohan, None; J.S. Lee, None; R. Ambrósio, None; A.E.K. Hutcheon, None; J. Zieske, None. Grant Identification: NIH grant EY 10056 and Res to Prevent Blindness
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2931. doi:
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    • Get Citation

      SE Wilson, RR Mohan, JW Hong, RR Mohan, JS Lee, R Ambrósio, AE K Hutcheon, J Zieske; Role of Epithelium in Myofibroblast Transformation in the Cornea . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2931.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To examine the effect of ectopic corneal epithelium on keratocyte apoptosis, keratocyte proliferation, and myofibroblast transformation. Methods: Eight-eight New Zealand white rabbits had lamellar flaps formed in one eye with a micokeratome. In two groups, a 3 mm circle of scraped donor epithelium (central or peripheral) was placed into the lamellar interface. No epithelium was placed into the interface in the control group. Animals were euthenized at 4 hours, 24 hours, 72 hours, 1 week, and 1 month. There were 4 eyes in each group at each time point, except there were 8 in each group at the 4-hour time point. Stromal cell apoptosis was monitored with the TUNEL assay. Epithelial tissue, stromal cell proliferation, and myofibroblast generation were monitored with immunocytochemistry for keratin K3, Ki-67, and alpha smooth muscle actin, respectively. Results: Epithelium expressing keratin K3 was detected in the interface at all time points in groups in which central or peripheral epithelium was introduced. Keratocyte apoptosis at 4 hours was 15±1 (SEM) (p < 0.0001), 21±1 (p < 0.0001), and 9±1 in the central epithelium, peripheral epithelium, and control groups, respectively. Keratocyte proliferation at 72 hours was 29±30 (p < 0.05), 30±12 (p < 0.05), and 16±6 in the central epithelium, peripheral epithelium, and control groups, respectively. At 1 week and 1 month after surgery, myofibroblasts were present at the level of the lamellar interface in the central epithelium and peripheral epithelium groups, but not in the control group. Conclusions: Epithelial-stromal interactions are critical in the modulation of keratocyte apoptosis, keratocyte proliferation, and myofibroblast generation in corneal wound healing. 

Keywords: 374 cornea: stroma and keratocytes • 380 cytokines/chemokines • 340 cell-cell communication 
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