December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Early Results of Ocular Surface Reconstruction in Unilateral Severe Limbal Stem Cell Deficiency Using Autologous Cultured Limbal and Conjunctival Stem Cells
Author Affiliations & Notes
  • VS Sangwan
    LV Prasad Eye Institute Hyderabad India
    Ocular Immunology
  • GK Vemuganti
    Ophthalmic Pathology Services LV Prasad Eye Institute Hyderbad India
  • S Singh
    Center for Cellular and Molecular Biology Hyderabad India
  • S Kashyap
    Ophthalmic Pathology Services
    LV Prasad Eye Institute Hyderabad India
  • G Iftekhar
    Ophthalmic Pathology Services
    LV Prasad Eye Institute Hyderabad India
  • GN Rao
    Cornea and Anterior Segment Services
    LV Prasad Eye Institute Hyderabad India
  • Footnotes
    Commercial Relationships   V.S. Sangwan, None; G.K. Vemuganti, None; S. Singh, None; S. Kashyap, None; G. Iftekhar, None; G.N. Rao, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2992. doi:
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    • Get Citation

      VS Sangwan, GK Vemuganti, S Singh, S Kashyap, G Iftekhar, GN Rao; Early Results of Ocular Surface Reconstruction in Unilateral Severe Limbal Stem Cell Deficiency Using Autologous Cultured Limbal and Conjunctival Stem Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2992.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To report the interim results of cultured limbal and conjunctival stem cell transplantation in patients with severe unilateral limbal stem cell deficiency (LSCD). Methods: Twenty five patients with total LSCD, secondary to chemical burns, unresponsive to standard medical and surgical treatments were chosen for cultured stem cell transplantation. Limbal tissue of 2x2 mm was harvested from the healthy eye and cultured by explant technique on de-epithelialized human amniotic membrane and transplanted to the ocular surface of the diseased eye, after excision of pannus. Supplementation with autologous cultured conjunctival cells was done in 18 cases with severe conjunctival damage. The cases were followed up for a period of 1- 8 months. Results: The ocular surface stability and relief from symptoms was noted in all patients at 6 weeks follow-up. Of the 24 cases on regular follow-up, 18/24 (75 %) cases showed stable ocular surface at the last follow-up. Two of these cases underwent successful keratoplasty with good visual recovery. Two cases (8.3 %) failed with recurrence of vascularization. Supplementation with conjunctival cell cultures provided a healthy bed for limbal transplantation in all cases; with restoration of the entire surface in 17/18 cases. Conclusions: Cultured limbal cell transplantation is helpful in restoring ocular surface stability in most cases of limbal stem cell deficiency. In severe LSCD with associated damage to conjunctiva, simultaneous transplantation of cultured conjunctival cells is a novel method of restoring the entire ocular surface integrity.

Keywords: 369 cornea: clinical science • 365 conjunctiva • 607 transplantation 
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