December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Fluorophore Assisted Retinal Break Detection: In vivo Studies Using Cy3 Tagged Antibody to Glial Fibrillary Acidic Protein
Author Affiliations & Notes
  • TL Jackson
    Ophthalmology Rayne Institute London United Kingdom
  • J Marshall
    Ophthalmology Rayne Institute London United Kingdom
  • Footnotes
    Commercial Relationships   T.L. Jackson, None; J. Marshall, None. Grant Identification: Special Trustees St Thomas' Hospital, Anne Allerton Trust
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3001. doi:
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      TL Jackson, J Marshall; Fluorophore Assisted Retinal Break Detection: In vivo Studies Using Cy3 Tagged Antibody to Glial Fibrillary Acidic Protein . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3001.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To demonstrate that fluorophore assisted retinal break detection is possible using an in vivo model of rhegmatogenous retinal detachment(RRD) Methods: Intubated hybrid pigs underwent 3-port pars plana vitectomy with creation of a RRD by subretinal injection of dilute viscoelastic. Cy3 anti-GFAP was injected into the vitreous cavity at the time of initial surgery, or as follow-up operations, and then rinsed from the eye. Excitation illumination was provided by a 532nm diode pumped laser transmitted to the eye via a standard endoscopic fibre-optic cable. Barrier filters were fitted to the operating microscope. Results: Consistent with our previous ex vivo studies, Cy3 anti-GFAP selectively labelled exposed glial cells at the margin of retinal breaks. Retinal breaks that were not evident using standard white-light endo-illumination were clearly visible when viewed through barrier filters, and illuminated with laser-light. There was negligible background staining, and confocal imaging of excised retinal breaks indicated a signal-to-noise ratio in excess of 250. Conclusion: This study confirms that Cy3 anti-GFAP can be used peroperatively to enhance the detection of retinal breaks.

Keywords: 563 retinal detachment • 479 Muller cells 
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