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E Hoyama, SA Schellini, ME A Marques, R Rossa, CR Padovani; Acellular Dermal Graft Study: A comparison between porcine and human derived implants in a rat model . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3028.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:The purpose of this study was to evaluate the host response of a porcine (P) and a human (H) derived acellular dermal graft (ADG) implanted in the subcutaneous tisuue of a rat model. Methods:Two subcutaneous pockets were surgically created along the dorsal midline of 25 rats (5 rats/group). The HADG was placed superiorly and the PADG, inferiorly. The animals were sacrificed at 07, 15, 30, 60 and 180 postoperative days (PO) and the ADGs and surrounding soft tissues (SST) were assessed for histopathological evaluation. The host response was quantified using an image analysis system to count the numbers of neovascularization per area of ADG and SST, and to verify the implant absorption rate by determining the total area of vacuoles inside the ADGs. The data were submitted to statistical analysis. Results:Light microscopy (LM) of PADG showed minimal inflammatory cell infiltrates (ICI) which increased until the 15 PO. A capsule formation (CF) surrounding the implant and regression of the ICI was observed 30 days after. At 60 PO the ICI and the CF around the implant persisted but were minimal. Vacuoles inside the PADG were observed at the 180 PO. The LM of the HADG revealed higher ICI than the observed in the PADG which also increased until the 15 PO. The CF surrounding the implant and regression of the ICI was also observed at the 30 PO, but the vacuoles inside the HADG were already present after the 60 PO and increased until the 180 PO. The HADG elicited a higher inflammatory response than the PADG during all experimental moments. The implant absorption rate was also higher in the HADG and earlier observed than in the PADG. Conclusion:Both PADG and HADG elicited minimal to moderate inflammatory response being inert and partially integrated to the SST, but presented some rate of absorption when implanted in the subcutaneous tissue of rats. The PADG showed to be a good alternative to be used as an implant material.
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