December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
A Comparison of Treatments for Lysozyme Removal from High Water Ionic Contact Lenses
Author Affiliations & Notes
  • MT Christensen
    Consumer Products Clinical Alcons Labs Inc (R6-15) Fort Worth TX
  • DJ Keith
    Alcon Research LTD Fort Worth TX
  • RE David
    Consumer Products Clinical
    Alcon Research LTD Fort Worth TX
  • Footnotes
    Commercial Relationships    M.T. Christensen, Alcon Research LTD. E; D.J. Keith, Alcon Research LTD E; R.E. David, Alcon Research LTD E.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3080. doi:
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      MT Christensen, DJ Keith, RE David; A Comparison of Treatments for Lysozyme Removal from High Water Ionic Contact Lenses . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3080.

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Abstract

Abstract: : Purpose:A comparison of a rub/rinse/soak with a phosphonate based MPS to a no-rub/rinse/soak and a no-rub/no-rinse/soak with a citrate based MPS in removing lysozyme. Methods:Acuvue 2 lenses were collected from 18 subjects in a masked 3 period crossover study (period = 7 days). Lenses were worn on a daily wear basis using one of the following two solutions: a phosphonate based MPS or a citrate based MPS per label instructions. On Day 7 all OD lenses (representing soiled lenses) were put directly into extraction fluid for HPLC analysis. The OS lenses (representing cleaned lenses) were assigned to one of the following treatments prior to HPLC analysis: Treatment A - Rub/Rinse/Soak with phosphonate based MPS. Treatment B - No-Rub/Rinse/Soak with citrate based MPS and Treatment C - No-Rub/No-Rinse/Soak with citrate based MPS. Results:Lenses treated using the phosphonate MPS with a Rub/Rinse/Soak treatment showed lysozyme levels of ∼1290 µg in the soiled lenses (OD) and ∼1250 µg in the cleaned lenses (OS). Lenses treated using the citrate based MPS with a No-Rub/Rinse/Soak treatment showed lysozyme levels of ∼1020 µg in the soiled lenses (OD) and ∼760 µg in the cleaned lenses (OS). Lenses treated using the citrate based MPS with a No-Rub/No-Rinse/Soak treatment showed lysozyme levels of ∼960 µg in the soiled lenses (OD) and ∼740 µg in the cleaned lenses (OS). Conclusion: The data shows that the phosphonate based MPS with a Rub/Rinse/Soak removed minimal lysozyme (2.9%). The citrate based MPS with a No-Rub/Rinse/Soak removed 25.5% total lysozyme. The citrate based MPS with a No-Rub/No-Rinse/soak removed 23.4% total lysozyme. Elimination of the rinse from the citrate based MPS treatment showed little affect on the total amount of lysozyme removed. This study was sponsored and conducted by Alcon Research Ltd, Fort Worth, TX.

Keywords: 367 contact lens • 358 clinical laboratory testing • 359 clinical research methodology 
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