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I-J Wang, C-Y Wu, F-R Hu, P-T Hung; Effect of Inflammatory Cytokines on the Regulation of Muc5ac Gene Expression . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3157.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To investigate the regulation of Muc5AC gene expression by different kind of cytokines. Methods:Human Muc5AC promoter with a luciferase reporter gene was transfected into a human conjunctival epithelial cell by lipofectin. Then,the promoter activity was challenged with IL-1b, IL-2, IL-6, IL-8 and TNF-a respectively. In vivo assay of Muc5AC was performed by gene gun delivery of plasmid-coated gold particles into rabbit conjunctiva. Results:The Muc5AC reporter activity could be affected by these pro-inflammatory cytokines in vitro and in vivo.Stimulation of transfected human conjunctival cell line with TNF-a and IL-1b resulted in a two-fold and four- to sixfold increase in Muc5AC promoter activity respectively. In vivo assay also revealed a several-fold increase in Muc5AC promoter activity in transtected rabbit conjunctival tissue after being challenged with different cytokines,especially with TNF-a, IL-2 and IL-8. Conclusion:The secretion of secretary mucin 5 was affected by inflammatory cytokines. Our results imply that decreasing the ocular surface inflammation may play an important role in the treatment of ocular surface disorder, such as dry eye syndrome.
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