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H Fujishima, T Terashima, N Asano-Kato, N Okada, Y Takano, A Igarashi, K Tsubota, K Fukagawa, H Saito; Diesel Exhaust Particles Elicit an Allergic Proinflammatory Response in Human Conjunctival Epithelium . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3169.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Diesel exhaust particles (DEP) from diesel engines, has been reported to be an important environmental factor in the pathogenesis of allergic inflammation. In order to clarify the role of DEP in allergic conjunctivitis, we investigated the induction of mRNA from conjunctival epithelial cells using the gene chip method. Methods:Primally cultures of human conjunctival epithelium were established in culture as previously described and then cultured with/without DEP (sonicated by ultrasound sonicator(Biorupture) for 6 hours). 1,000,000 epithelial cells were extracted and treated for DNA extraction. The expression of approximately 12,000 mRNA were examined by gene chip. Results:The adhesion molecule ICAM-1, the cytokines IL-1beta and IL-6, the chemokines, LARC, RANTES, CCR-5, GRO-alpha, -beta, and IL-8, and the growth factors ECGF and EGF were unregulated. Conclusion:DEP induced the expression of proteins that are important in the growth and survival of mast cells. DEP-induced proinflammatory response in conjunctival epithelial cells may play a crucial role in allergic conjunctivitis.
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