December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Gene and Protein Expression of ET-A and ET-B Receptor in Normal and Vascularized Human Corneas
Author Affiliations & Notes
  • A Kuhlmann
    Department of Ophthalmology University of Erlangen-Nuernberg Germany
  • C Cursiefen
    Department of Ophthalmology University of Erlangen-Nuernberg Germany
  • K Amann
    Department of Pathology University of Erlangen-Nuernberg Germany
  • M Küchle
    Department of Ophthalmology University of Erlangen-Nuernberg Germany
  • U Schlötzer-Schrehardt
    Department of Ophthalmology University of Erlangen-Nuernberg Germany
  • Footnotes
    Commercial Relationships   A. Kuhlmann, None; C. Cursiefen, None; K. Amann, None; M. Küchle, None; U. Schlötzer-Schrehardt, None. Grant Identification: IZKF Erlangen(B13)
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3199. doi:
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      A Kuhlmann, C Cursiefen, K Amann, M Küchle, U Schlötzer-Schrehardt; Gene and Protein Expression of ET-A and ET-B Receptor in Normal and Vascularized Human Corneas . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3199.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Endothelin (ET) A and B receptors are targets of ET-1 which is a potent mitogen for endothelial cells and an inducer of angiogenesis. ET-1 has been detected in newly formed pathologic blood vessels in human corneas. We therefore analyzed mRNA and protein expression of ET-A and ET-B receptors in normal non-vascularized and vascularized human corneas. Methods: Using in situ-hybridisation, indirect immunohistochemistry and double immunofluorescence (with antibodies against CD 31), we investigated ET-A and ET-B receptor mRNA and protein expression in normal corneas, in corneas with keratoconus and Fuchs' endothelial dystrophy, and in vascularized corneas secondary to graft rejection, trauma and herpetic keratitis. Results: Normal human corneas displayed ET-A and ET-B receptor mRNA and protein expression in the basal layers of the corneal epithelium and corneal endothelium. Whereas ET-A receptor was found predominantly in the cytoplasm of epithelial cells, keratocytes and endothelial cells, ET-B receptor showed a nuclear staining pattern in epithelial and endothelial cells. The keratocytes showed almost no reactivity for ET-B receptors. Expression of ET-A and ET-B receptor mRNA and protein was more pronounced in the epithelium of vascularized compared to non-vascularized human corneas. The keratocytes of vascularized corneas showed a significantly increased ET-B receptor expression. In addition, ET-A and ET-B receptor mRNA and protein could be localized to endothelial cells of newly formed vessels by double labelling with anti-CD 31 antibodies. Conclusion: Expression of ET-A and ET-B receptors in endothelial cells of newly formed pathologic human corneal blood vessel as well as the upregulation of both receptors in the epithelium and ET-B in stromal keratocytes of vascularized corneas indicate involvement of ET-receptors in human corneal neovascularisation. Thus, ET-A and ET-B receptors may represent additional targets for an angiostatic therapy against corneal neovascularisation using well established receptor antagonists.

Keywords: 434 immunohistochemistry • 371 cornea: endothelium • 372 cornea: epithelium 
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