December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Regulatory Effect of Pilocarpine and/or Atropine on the Facility of Outflow in Perfused Bovine Anterior Eye Segments with Intact Trabecular Meshwork and Ciliary Muscle
Author Affiliations & Notes
  • AK Palkama
    Ophthalmology LSU Eye Center New Orleans LA
  • D Velasquez
    Ophthalmology LSU Eye Center New Orleans LA
  • Z Si
    Ophthalmology LSU Eye Center New Orleans LA
  • J Reynaud
    Ophthalmology LSU Eye Center New Orleans LA
  • HW Thompson
    Ophthalmology LSU Eye Center New Orleans LA
  • RW Beuerman
    Ophthalmology LSU Eye Center New Orleans LA
  • Footnotes
    Commercial Relationships   A.K. Palkama, None; D. Velasquez, None; Z. Si, None; J. Reynaud, None; H.W. Thompson, None; R.W. Beuerman, None. Grant Identification: EY12540, EY02377, RPB
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3270. doi:
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      AK Palkama, D Velasquez, Z Si, J Reynaud, HW Thompson, RW Beuerman; Regulatory Effect of Pilocarpine and/or Atropine on the Facility of Outflow in Perfused Bovine Anterior Eye Segments with Intact Trabecular Meshwork and Ciliary Muscle . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3270.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To analyze the regulatory effect of pilocarpine and/or atropine on perfused bovine anterior segments with intact trabeculum and ciliary muscle. Methods: For each bovine eye, the posterior 1/3 of the globe and the aqueous humor, lens, vitreous, and retina were removed and 4 peripheral iridectomies were performed. The resulting anterior segment was attached to a perfusion chamber while avoiding compression of the vortex veins. The chamber was connected to a reservoir monitored by an electronic balance and to a pressure transducer. The eyes were humidified at 37º C and were perfused from the reservoir at 11 mmHg with DMEM+PFS alone or with 10-9, 10-6, or 10-4 M pilocarpine (P) and/or 1.5x10-4 M atropine (A). Facility of outflow (µl/min/mmHg) was recorded continuously for 19 hr. Fluorescent microbeads (0.31, 0.9, 2.03, 2.6, 3.4, 4.2, or 5.1 µm diameter) were introduced into the anterior chamber at 19 hr and perfusion continued for 3 hr. Beads were collected from the perfusate outside the eye and diameters determined by fluorescence microscopy to evaluate pore sizes in the outflow pathways. Results: Facility of outflow is shown below (* indicates significant difference from control by analysis of regression).  

The effect of 10-4 M pilocarpine was totally blocked by atropine. In control eyes, the largest beads in the perfusate outside the eye were 2.03 µm in diameter. In the 10-4 P-treated eyes, 2.6-µm beads were seen in the perfusate outside the eye, but not in the ciliary muscle. Conclusion: Pilocarpine seems to have a dose-dependent contradictory effect on the facility of outflow. Pilocarpine also seems to regulate the pore sizes. Atropine increases the facility of outflow and totally blocks the effect of pilocarpine.

Keywords: 318 anterior segment • 503 outflow: trabecular meshwork • 514 pharmacology 
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