December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Nitric Oxide Donative Action of Nipradilol in the Retinal Microcirculation of Rats
Author Affiliations & Notes
  • K Tomida
    Department of Ophthalmology Nagoya City Univ Med School Mizuho-Ku Japan
  • Y Ogura
    Department of Ophthalmology Nagoya City Univ Med School Mizuho-Ku Japan
  • A Matsubara
    Department of Ophthalmology Nagoya City Univ Med School Mizuho-Ku Japan
  • Y Niwa
    Department of Ophthalmology Nagoya City Univ Med School Mizuho-Ku Japan
  • H Morita
    Department of Ophthalmology Nagoya City Univ Med School Mizuho-Ku Japan
  • K Tamai
    Department of Ophthalmology Nagoya City Univ Med School Mizuho-Ku Japan
  • Footnotes
    Commercial Relationships   K. Tomida, None; Y. Ogura, None; A. Matsubara, None; Y. Niwa, None; H. Morita, None; K. Tamai, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3287. doi:
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    • Get Citation

      K Tomida, Y Ogura, A Matsubara, Y Niwa, H Morita, K Tamai; Nitric Oxide Donative Action of Nipradilol in the Retinal Microcirculation of Rats . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3287.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:This study was designed to investigate a nitric oxide (NO) donative effect of topical nipradilol, an α1, ß-blocker, on the interactions in the retinal microcirculation. Methods:Male Brown-Norway rats were given NG-nitro-l-arginine methyl ester (L-NAME) in drinking water for 2 weeks to inhibit production of intrinsic NO in the retinal endothelium. Rats in group A received one eyedrop of 1% nipradilol twice daily. Rats in group B received one eyedrop each of 0.5% timolol and 0.125% bunazosin twice daily during the 2 weeks. Animals in group C had vehicle instillation only in the same way. Control animals were given drinking water without L-NAME and had vehicle instillation. The leukocyte velocity in the retinal capillaries and the number of leukocytes trapped in the retina were evaluated with acridine orange digital fluorography. Results:The number of trapped leukocytes was significantly greater in groups A, B and C than in control (22.78±0.36, 26.04±0.31, 27.13±0.55 and 5.43±0.26 cells/mm2, respectively, p<0.001). The rats received nipradilol showed less trapped leukocytes than the other groups (p<0.01).there were no significant differences in leukocyte velocities among all groups. Conclusion:The results suggest that the inhibition of NO production causes a disturbance of leukocyte circulation in the retina and that topical nipradilol appears to have a NO donative effect of alleviating leukocyte-endothelial interaction in the retinal microcirculation.

Keywords: 390 drug toxicity/drug effects • 491 nitric oxide • 554 retina 
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