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SB Pressmar, J Carstens, V Sturm, E Pörksen, N Schmitz, G Richard, F Fankhauser; Quantification of Argon-Laser-induced damage of the blood-retinal-barrier: In vivo measurement of vitreal molecular changes by means of Dynamic Light Scattering (DLS) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3298.
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Purpose: Dynamic Light Scattering (DLS) is an established non-invasive method for in vivo measurement of protein changes within the vitreous. In this study we set to quantify molecular changes of the protein structure of the vitreous before and after Argon-Laser-retinopexy. Methods: A collective of 10 patients with non-proliferative diabetic retinopathy grade 1 - 3, who never had laser treatment of the retina before, was examined with DLS. Measurements were performed before, immediately after, as well as at day 1, 5, 14 and week 4, 6, 8 after laser treatment. Results: Immediately after laser treatment there was an inflow of plasma proteins into the vitreous indicated by a shift of the autocorrelation functions towards higher frequencies. During the follow-up period the autocorrelation functions showed a trend to return to the preoperative state within an interindividually variable time-interval of 4 to 8 weeks. Conclusion: In this study we have demonstrated, that DLS is a suitable tool for detecting the breakdown of the blood-retinal-barrier after Argon-Laser-retinopexy. Due to the limited number of patients and a marked interindividual variability in this pilot-study the observed trends did not reach statistically significant levels, which are currently under investigation at a larger collective.
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