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O Yamanaka, S Saika, Y Ohnishi, A Ooshima; Role Of Connective Tissue Growth Factor In Extracellular Matrix Components Production In Cultured Human Subconjunctival Capsule Fibroblasts And Their Proliferation And Migration . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3341.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Subconjunctival fibroblasts (SFs) are believed to be responsible for bleb scarring following filteration surgery. Connective tissue growth factor (CTGF) is a cytokine recently focused in tissue fibrosis. Here to know the roles of CTGF in bleb scarring, effects of CTGF antisense-deoxynucleotide (antisense) on the cell proliferation, extracellular matrix production and wound closure in monolayer cell sheet were examined in SF cultures in the presence or absence of TGFbeta1. Methods:The effects of CTGF antisense on cell proliferation and closure of a linear defect produced in a SF monolayer sheet were examined. Concentrations of collagen type I and fibronectin in culture medium and cell lysate in the presence or absence of CTGF antisense and/or TGFbeta1 were determined using enzyme immunoassay kits. Results:CTGF antisense inhibited cell proliferation and decreased production of collagen and fibronectin. Inhibition of ECM production by CTGF antisense was larger in SFs treated with exogenous TGFbeta1 than in non-treated cells. Adding CTGF antisense reduced the ratio of in vitro wound closure. Conclusion:CTGF plays an important role in ECM production by SFs. Reduced production of ECM might perturb cell proliferation and migration. TGFbeta1-stimulation of ECM production is in part attributed to endogenous CTGF induction. Inhibition of TGFbeta1-enhanced CTGF expression may be effective to prevent undesirable scar formation following filteration surgery.
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