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M Maruichi, T Sugiyama, S Takai, M Ueki, M Sakaguchi, M Miyazaki, T Ikeda; Effect of Chymase Inhibitor on Postoperative Wound Healing After Trabeculectomy in Canine Eyes . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3347.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To compare the effect of chymase inhibitor (CI) on the wound healing after trabeculectomy with that of mitomycin C(MMC). Methods: Trabeculectomy was performed with MMC 0.4mg/ml or CI (Suc-Val-Pro-PheP(Oph)2) 10µmol/L or physiological saline in one of the eyes of 6 normal dogs, while the other eyes served as controls. On the 14th postoperative day, eyes were enucleated and subjected to a pharmacological or histological evaluation. The activities of chymase and angiotensin converting enzyme were measured in the treated sites of conjunctives. Filtering blebs were also examined histologically after Azan staining. Results: The chymase activities in filtering blebs of MMC-, CI- and physiological saline- treated operative eyes were 3.594, 1.457 and 8.933 mU/mg protein, respectively. Collagen fibers were sparse and vascular cells were rich in the filtering blebs of CI-treated eyes, as compared to MMC-treated eyes. Conclusion: The results suggested that CI might prevent postoperative bleb adhesion without making cystic or thin-walled filtering blebs which are susceptible to infection. Therefore CI might replace MMC as the agent used in trabeculectomy procedures.
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