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CS Mayer, G Kroher, K Kobuch, D Spiegel; Location and Correlation of PEX Material in Patients With Primary Open Angleglaucoma or Pseudoexfoliation Glaucoma . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3399.
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Purpose: Pseudoexfoliation (PEX) material is a non fully identified polypeptide often associated with secondary open angle glaucoma. In this study we want to evaluate the location of PEX material in iris speciments and to correlate PEX material with the clinical findings of PEX syndrome in patients with glaucoma. Methods: We evaluated 61 glaucoma patients treated with trabeculectomy. One group has visible PEX material on iris, pupil and/or lens. In the other group was no PEX material preoperative detectable. To determine the location of PEX material in the iris we used the purified monoclonal mouse-anti-human-IG CD47/HNK-1 clone VC1.1 conjugated with FITC on iris speciments using fluorescence microscopy after embedding in PFA. The findings of histology were correlated to the clinical evaluation regarding visible PEX material in the eye. Results: In 20% (17/61) of all investigated patients PEX material was clinical visible and could be also found in immunfluorescence microscopy. PEX material was located in 50% next to vessels and stromal strands and 50% elsewere in the iris speciments. No PEX material in clinical examination and in immunology were found in 43% (26/61) of all investigated patients. In 16% (10/61) PEX material only could be detected in immunfluorescence microscopy. 18% (11/61) with clinical visible PEX material were negative tested in immunhistology. Conclusion: PEX material in iris speciments could be found in all iris structures. Since finding of PEX material in the iris does not strictly correlate with the clinical finding, different types, expression location or state of expression of the PEX material might be discussed.
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