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H Choi, J-T Kim, C-K Joo; Effect of Insulin on Epithelial-Mesenchyme Transition(EMT) Iinduced by TGF-beta in Bovine Lens Explants . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3545.
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Purpose: Epithelial-mesenchyme transition(EMT) is a dynamic process in which epithelial cells transit to mesenchymal cells. Transforming growth factor (TGF)-beta is a multifunctional cytokine, that is well known to induce EMT. Our previous studies showed that TGF-ß induced the expression of mesenchymal marker in lens epithelial cells (LECs). The purpose of this study is to examine a role of insulin in EMT of bovine lens induced by TGF-beta. Methods: We cultured bovine lens epithelial explants in MEM containing 0.2% bovine serum albumin at 37°C for 12 hrs and treated them with 10ng/ml TGF-beta or 100ng/ml insulin for 24hrs. For analysis of extracellular matrix (ECM) protein and alpha-smooth muscle actin (SMA) we utilized reverse transcription-polymerase chain reaction (RT-PCR) and western blot analyses. Results: The protein and RNA levels of fibronectin and alpha-SMA were increased after TGF-beta treatment, whereas these increases were inhibited by insulin. Conclusion: Insulin may play a role of inhibition of EMT induced by TGF-beta.
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