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DL Boyle, L Takemoto, JP Brady, EF Wawrousek; Morphological Characterization of the alphaA-/ alphaB-Crystallin Double Knockout Mouse Lens . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3582.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Characterize the gross lenticular morphology, by scanning electron microscopy (SEM) and confocal microscopy, of normal mice and mice with targeted disruption alphaA- and alphaB-crystallin genes. Methods: Lenses from 129SvEvTac mice (wild type) and mice with the targeted disruption of alphaA- and alphaB-crystallin genes were examined by standard scanning electron microscopy and confocal microscopy. Results: Equatorial and axial (sagittal) dimensions of lenses for alphaA/BKO mice were significantly smaller than age-matched wild type lenses. No posterior sutures or fiber cells extending to the posterior capsule were found in alphaA/BKO lenses. Aberrant nucleic acid staining was observed in the posterior subcapsular region of 5 wk and anterior subcapsular cortex of 54 wk alphaA/BKO lenses. Gross morphological differences were observed in the equatorial, posterior and anterior regions of lenses from alphaA/BKO mice as compared to wild type mice. Conclusions: The results, in conjunction with previous studies (Brady et al., 2001), indicate that predominately alphaA-crystallin, and to a lesser extent, alphaB-crystallin, are necessary for lens transparency, and may be essential for proper fiber cell formation.
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