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Q Chu, TT Lam; Retinal Ischemia-Reperfusion Induced Changes In Protein Expression In Rat Retinas . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3678.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Retinal ischemia-reperfusion induced apoptosis, a highly regulated process of cell death. The exact pathways of cell death in this system remain to be examined. Our aim is to identify the proteins involved in neuronal cell death and survival. Methods: Ischemia-reperfusion was induced by a previously described procedure using elevated intraocular pressure for 60 minutes. Rat retinas at 8 hours after the insult were collected and the total proteins were extracted in ampholyte containing buffer. Two dimensional (2-D) gel electrophoresis with a pH gradient of 3 to10 was conducted to examine the protein changes induced by ischemia-reperfusion. Protein spots were visualized with silver staining and Commassie blue staining. Results: Reproducible profiles were noted in the normal and the injured retinas. Comparing the normal and the ischemia-reperfused retinas, two prominent spots at 50 kDa and 70 kDa with alkali pIs were upregulated while another prominent spot at 50 kDa with acidic pI was downregulated plus several new but minor spots appeared and several minor spots disappeared in the ischemia-reperfused retina. Conclusion: Ischemia-reperfusion injury affects the protein profiles of the retinal tissues. Further studies are underway to identify proteins related to cell death and/or cell survival.
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