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D Ma, HJ Wu, RJ F Tsai, JK Chen, LK Yeh; Inhibition of Capillary Morphogenesis In Vitro by Ex Vivo Expanded Limbal Epithelial Cells Cultivated on Preserved Human Amniotic Membrane . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3718.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To understand the anti-angiogenic effect of ex vivo expanded human limbal epithelial cells (HLE) cultivated on preserved human amniotic membrane (HAM). Methods: Human umbilical vein endothelial cells (HUVEC) were seeded in type I collagen gel in a transwell, which was in turn cocultured with human limbal fibroblasts (HLF) seeded in three-dimensional type I collagen gel containing (1) no HAM (2) HAM only (3) HLE cultivated on HAM (HLE/HAM). After three days, total capillary tube length was measured and analyzed by digital image analyzer. Results: HLF in type I collagen gel induced prominent capillary tube formation by HUVEC. Capillary tube length in the HLF gel containing only HAM group was only mildly decreased. However, capillary tube length in the HLF gel containing HLE/HAM group was significantly reduced. Conclusion: HAM alone express anti-angiogenic activity in vitro, and this effect may be further augmented by HLE, which may be responsible for the clinical anti-angiogenic/anti-inflammatory effect of HLE/HAM transplantation.
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