December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Do Intraflagellar Transport Proteins Assemble into a Complex in the Vertebrate Retina?
Author Affiliations & Notes
  • SA Baker
    Cell Bio/Neurobio & Anatomy Medical College of Wisconsin Milwaukee WI
  • G Pazour
    Worcester MA
  • D Cole
    Moscow ID
  • G Witman
    Worcester MA
  • JC Besharse
    Cell Bio/Neurobio & Anatomy Medical College of Wisconsin Milwaukee WI
  • Footnotes
    Commercial Relationships   S.A. Baker, None; G. Pazour, None; D. Cole, None; G. Witman, None; J.C. Besharse, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3742. doi:
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      SA Baker, G Pazour, D Cole, G Witman, JC Besharse; Do Intraflagellar Transport Proteins Assemble into a Complex in the Vertebrate Retina? . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3742.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Intraflagellar Transport (IFT) has been identified in motile flagella of Chlamydomonas and sensory cilia of C. elegans as a mechanism required for assembly and maintenance of cilia and flagella. In IFT, movement of a large complex of ∼17 IFT proteins along axonemal microtubules is catalyzed by Kinesin II and cytoplasmic dynein. Our goal is to use biochemical and immunological techniques to study IFT protein interactions in the mammalian retina. Methods: Indirect immunocytochemistry was performed on fresh frozen bovine retinal sections. Bovine retinal extracts were prepared by homogenization in HMEK buffer. Ciliary axonemes were prepared by detergent extraction of bovine RIS/ROS. Extracts were fractionated on sucrose density gradients and analyzed by western blotting with antibodies raised against the mouse sequences of IFT20, IFT52, IFT57, and IFT88. Results: Immunocytochemistry reveals that each of the four IFT proteins is found throughout the inner segment and concentrated at the basal body-connecting cilum. When retinal extracts are analyzed on sucrose density gradients we find that the IFT proteins are broadly distributed from 21S-3S, suggesting a large monomeric pool of IFT proteins as well as larger protein complexes. An additional pool of IFT proteins tightly associates with the ciliary axoneme and requires harsh chaotropic salts or ionic detergents for extraction. Conclusion: Our data suggest that IFT20, 52, 57, and 88 interact as part of a protein complex as predicted by the IFT model. However, a significant portion of IFT protein in retina exists in both a monomeric pool and in a pool that is bound in a stable fashion to the photoreceptor cytoskeleton.

Keywords: 517 photoreceptors • 561 retinal degenerations: cell biology 
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