December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Transferrin , Transferrin Receptor and Ferritin in Human Retina
Author Affiliations & Notes
  • YG Courtois
    Dev Aging/Pathology the Retina Inserm U450 Institut des Cordeliers Paris France
  • J-CP B Jeanny
    Dev Aging/Pathology the Retina Inserm U450 Institut des Cordeliers Paris France
  • C Sergeant
    Umr 5084 CNRS Bordeaux-Gradignan France
  • M Valtink
    Department of Ophthalmology Cornea Bank Hamburg Germany
  • M Yefimova
    Neurochemistry Sechenov Institut of Evolutionary Physiology and Biochemistry St-Petersburg Russian Federation
  • Footnotes
    Commercial Relationships   Y.G. Courtois, None; J.P.B. Jeanny, None; C. Sergeant, None; M. Valtink, None; M. Yefimova, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3744. doi:
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      YG Courtois, J-CP B Jeanny, C Sergeant, M Valtink, M Yefimova; Transferrin , Transferrin Receptor and Ferritin in Human Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3744.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To describe the immunolocalization of the main iron homeostasis proteins in human retina. Iron is an essential microelement, but disruption of iron homeostasis could contribute to neurodegenerative disorders. Methods: Fixed or unfixed frozen sections, 5µm thick, were prepared from enucleated eyes of humans 64 to 72 years old. The sections were used to analyse by immunohistochemistry the distribution of transferrin, transferrin receptor and ferritin, using specific monoclonal and polyclonal antibodies. Results: The weak anti-transferrin immunostaining was observed throughout the neural retina. The main site of transferrin immunolocalization in human retina is the outer and inner segments of the photoreceptor cells. The presence of transferrin in retinal pigmented epithelium was difficult to document due to autofluorescent granules. Transferrin receptor protein is widely distributed in human retina. The very pronounced immunostaining was observed in retinal pigmented epithelium, few cells of the inner nuclear layer, both outer and inner segments of photoreceptors and ganglion cell layer, more precisely, at the level of the nerve fiber layer. The strongest immunoreactivity for ferritin was attributed to retinal pigmented epithelium, inner segments of photoreceptors, inner and outer plexiform layers and ganglion cell layer. Studies are also in progress to describe by PIXE (proton induced X-ray emission) the iron distribution in human retina. Conclusion: The distribution of main iron homeostasis proteins in human retina follows the same pattern as we have already described for bovine and rodent retina (Yefimova et al., Invest. Ophthalmol. Vis. Sci., 2000 ; 41:2343-51). The data obtained suggest that many aspects of retinal iron homeostasis could be common in different mammals.

Keywords: 434 immunohistochemistry • 554 retina • 567 retinal pigment epithelium 
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