Purchase this article with an account.
HI Salti, AC Clermont, E Duh, L Goddard, LP Aiello; Diabetes-Induced Retinal Vascular Permeability is Reversed by Intravitreal Injection of Pigment Epithelial Derived Factor . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3864.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Previously, we demonstrated that Pigment Epithelial Derived Factor (PEDF) blocks VEGF-induced retinal vascular permeability in vivo in a dose dependent manner. Since an increase in VEGF expression and retinal vascular leakage is associated with diabetic retinopathy and diabetic macular edema, we investigated whether exogenously added PEDF could inhibit diabetes-induced retinal vascular permeability. Methods: Retinal vascular leakage in non-diabetic and STZ-induced diabetic Sprague-Dawley rats was measured by the Evans Blue albumin permeation technique. At days 11 and 13 after induction of diabetes, 10 ul of PEDF (200 ng/eye, 40nM final) was injected into one eye and vehicle (0.1% BSA in PBS) was injected into the contralateral eye under sterile conditions. The intact condition of each eye and retina was assessed 24 hours after each injection. At day 14, the animals were sacrificed and retinal vascular permeability was determined. Results: Vascular leakage was increased by 79% (p=0.026) in diabetic rats (7.7±3.4, n=11) as compared with non-diabetic rats (4.3±1.6, n=7). PEDF suppressed diabetes-induced retinal vascular leakage by 88% (4.7±3.8, n=7, p=0.031). Vascular permeability was not significantly altered in non-diabetic rats treated with PEDF (3.7±1.0, n=7). Conclusion: Intravitreal injection of PEDF significantly normalized diabetes-induced retinal vascular leakage within three days even after eleven days of diabetes. Durability of this effect is under investigation. These data suggest that PEDF may have significant therapeutic potential for the treatment of diabetic macular edema and other VEGF-related disorders.
This PDF is available to Subscribers Only