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A-MM Demetriades, PL Gehlbach, S Yamamoto, T Deering, EW Ng, I Kovesdi, D McVey, L Wei, PA Campochiaro; Periocular Injection of an Adenoviral Vector Expressing the Extracellular Portion of Aascular Endothelial Growth Factor Receptor-1 (Adsflt.10) Inhibits Choroidal Neovascularization (CNV) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3916.
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Purpose: To determine the effect of periocular injection of Adsflt.10 on CNV after laser-induced rupture of Bruch’s membrane in mice. Methods: Bruch’s membrane was ruptured with laser photocoagulation at three locations in each eye of adult C57BL/6 mice. Immediately after laser treatment, mice were given a periocular injection of 3µl containing 5x109 particles of Adsflt.10 in one eye and 5x109 particles of corresponding control vector (Adnull.10) in the fellow eye, or 5µl containing 2.33x1010 particles of Adsflt.10 in one eye and 2.33x1010 particles of Adnull.10 in the fellow eye. After two weeks, mice were perfused with fluorescein-labeled dextran and the area of CNV at each rupture site was measured on choroidal flat mounts by computerized image analysis. Results: Compared to fellow eyes that received periocular injection of 5x109 particles of Ad.null.10 (CNV area = 0.03740.0106 mm2, n=21), the mean area of CNV at Bruch’s membrane rupture sites was significantly reduced in eyes given a periocular injection of 5x109 particles (n=32; CNV area = 0.00980.0017 mm2; p=0.015) or 2.33x1010 particles of Adsflt.10 (n=21; CNV area = 0.0087 0.0024 mm2, p=0.013). The difference between low and high dose Adsflt.10 was not significant (p=0.728). Conclusion: Periocular injection of Adsflt.10 inhibits CNV in the ipsilateral, but not the contralateral eye implicating local delivery of sflt in the inhibitory effect.
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