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N Callizot, A-C Fintz, CL Percicot, M Simonutti, J Sahel, GN Lambrou; Experimental Model for Optic Nerve Protection Studies . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4049.
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Purpose: To develop a model of optic nerve degeneration in adult mouse mice and assess the effect of the lesion with electrophysiology and histology. Methods: Unilateral optic nerve crushes were performed with a self-closing gentle tensioned forceps in 10 weeks-old C57Bl/6J male mice. Control group were sham-operated. Binocular and monocular recordings of visually evoked potentials (VEP) signals were performed. Latency and amplitude of VEP were studied. Number of optic nerve fibers were estimated 4 weeks after the crush in both group. Results: No significant differences were observed in the bilateral recordings of VEP. Latencies slightly increased and amplitudes of the signal were significantly weaker in crushed compared to sham-operated eyes from 10 days after the crush in monocular recordings. Numbers of myelinized fibers were significantly reduced in crushed nerve as in the centre as in the periphery of the nerve. Conclusion: The results with VEP reflected a visual impairment which is confirmed by histological analysis of the optic nerve fibers number. This experimental nerve degeneration is suitable for in vivo studies of optic nerve protection especially in the case of glaucoma.
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