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M Nakamura, T Nagano, K Nakata, Y Ohashi; Effects of Levofloxacin on Corneal Epithelial Cell Proliferation and Migration, and Keratocyte Proliferation in vitro . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4216.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: We investigated the effects of various kinds of quinolones, levofloxacin (LVFX), ofloxacin (OFLX), norfloxacin (NFLX), and lomefloxacin (LFLX) on corneal epithelial cell proliferation and migration, and keratocyte proliferation in vitro. Methods: For cell proliferation experiments, human epithelial cells and rabbit keratocytes were cultured and treated with quinolones for 3 days. After that, the proliferative activity was measured by MTS assay system. For cell migration experiments, cultured rabbit corneal blocks were treated with quinolones for 24 hours after which the length of the path of corneal epithelium was measured. Results: LVFX, OFLX, NFLX, and LFLX inhibited epithelial cell and keratocyte proliferation in a dose-dependent manner (0.01-1 mg/ml). The inhibitory effect of NFLX on epithelial cell and keratocyte proliferation was observed at the lowest concentration tested. Among the quinolones tested, NFLX was the most potent inhibitor followed by LFLX. The inhibitory effects of LVFX and OFLX were similar, and were observed at the highest concentrations tested. The same order of inhibitory strength of the tested quinolones was obtained when the effect on epithelial cell migration was examined. Conclusion: The present results suggest that LVFX has inhibitory effects on corneal epithelial cell and keratocyte proliferation and epithelial migration comparable to OFLX, and less than LFLX and NFLX.
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