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T Kato, J-H Chang, DT Azar; Expression of Type XVIII Collagen During Wound Healing of Corneal Incisions and Keratectomy Wounds . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4231.
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Purpose:: To determine the distribution of type XVIII collagen in mouse ocular tissues and to investigate the expression of type XVIII collagen during corneal wound healing of corneal incisions and keratectomy wounds. Methods:We performed immunohistochemical analysis of type XVIII collagen in mouse ocular tissue using polyclonal antibodies to the hinge domain. For wound healing experiment, we performed excimer laser keratectomy and single linear incisions on mouse corneas. The corneas were harvested at various times after wounding and processed for either immunohistochemistry, in situ hybridization, competitive polymerase chain reaction (PCR) or western blotting. Results:In the mouse ocular tissues, type XVIII collagen was immunolocalized to the corneal epithelium, ciliary epithelium, lens capsule, retinal internal limiting membrane, and Bruch’s membrane. After excimer laser keratectomy, type XVIII collagen was expressed in the corneal epithelium. After lineal corneal incisions type XVIII collagen was also expressed in the corneal epithelium. In addition, type XVIII collagen was immunolocalized to the subepithelial stromal wound healing region 1 week after wounding and its mRNA showed corresponding temporal increased expression in the same region. Conclusion:Our results suggest that type XVIII collagen is broadly expressed in ocular tissues and that it may have a potential role in wound healing after incisional corneal wounds.
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