December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Confocal Microscopy Findings In Deep Lamellar Keratoplasty
Author Affiliations & Notes
  • E Hernandez-Quintela
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • G de Wit-Carter
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • M Ramirez-Fernandez
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • M Hernandez
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • S Quintero
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • JC de-la-Luz
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • R Naranjo-Tackman
    Cornea Service Asociacion Para Evitar la Ceguera en Mexico UNAM Mexico City Mexico
  • Footnotes
    Commercial Relationships   E. Hernandez-Quintela, None; G. de Wit-Carter, None; M. Ramirez-Fernandez, None; M. Hernandez, None; S. Quintero, None; J.C. de-la-Luz, None; R. Naranjo-Tackman, None. Grant Identification: CONACyT Grant 115755
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4241. doi:
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    • Get Citation

      E Hernandez-Quintela, G de Wit-Carter, M Ramirez-Fernandez, M Hernandez, S Quintero, JC de-la-Luz, R Naranjo-Tackman; Confocal Microscopy Findings In Deep Lamellar Keratoplasty . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4241.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To describe confocal microscopy findings in deep lamellar keratoplasty (DLK). Methods:A central scan of full-thickness cornea was performed with a confocal microscope (Confoscan, Fortune Technology, Italy) in 4 eyes (4 patients) one week after DLK. Preoperatrive diagnosis included corneal ectasia after radial keratotomy (1), actinic keratoconjunctivitis (1), and granular dystrophy (2). The surgical procedure was performed with a 7.5 mm Barron trephine until desired depth was achieved, remaining stroma was delaminated with a crescent knife to Descemet’s membrane. Early morphology cellular findings after DLK are described. Results:Confocal microscopy examination of the donor cornea revealed anterior stromal keratocytes with evident cytoplasm and an increased reflectivity at a subepithelial level. Optical sections through the donor stroma showed microplicae arranged in a reticular pattern. The donor-receptor interface showed several highly reflective round images with 10 to 50 microns of diameter, with a disperse distribution. Confocal microscopy examination was able to show the endothelial mosaic in 2 cases. Conclusion:Confocal microscopy permitted in vivo visualization of cellular morphology changes of the cornea after DLK.

Keywords: 374 cornea: stroma and keratocytes • 631 wound healing • 471 microscopy: confocal/tunneling 
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