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H Alizadeh, M Hurt, VB Proy, JY Niederkorn; The Interaction of Acanthamoeba Castellanii Cysts with Macrophages and Neutrophils . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4292.
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Purpose:: To determine whether neutrophils and macrophages are able to kill Acanthamoeba cysts in vitro. Methods: Neutrophils and macrophages were collected from peritoneal cavities of naïve mice. The ability of murine macrophages and neutrophils to lyse Acanthamoeba cysts was determined by co-incubating these cells with Acanthamoeba cysts. Neutrophil and macrophage chemotaxis assays were performed using Transwell chambers. Results: Neutrophils and macrophages were capable of lysing a significant number of A.castellanii cysts in vitro (P<0.05). However, neutrophils were more efficient at killing cysts than macrophages. Macrophages and neutrophils were not chemotactically attracted to cysts. However, they displayed positive chemotactic response to the cyst lysate. Conditioned medium obtained from neutrophils induced significant cytolysis of A. castellanii cysts in vitro. Conclusion: The results indicate that neutrophils and macrophages can directly kill cysts and act as a first line of defense against Acanthamoeba keratitis.
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