Purchase this article with an account.
MR Glucksberg, FI Wu, K Shafer, R Van Duyne; Novel Nanoparticles For Contrast Enhancement Optical Coherence Tomography In Doppler . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4378.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To devise and characterize a biologically compatible contrast enhancing agent for Optical Coherence Tomography Methods: Degeneration of the choriocapillaris has been found in postmortem histologic analysis of choroids in patients afflicted with diabetes and age-related macular degeneration. Laser Doppler flowmetry has been successfully used to measure relative flow in the choroid in animals and humans, however, due to its poor spatial resolution use is limited to the foveal region which lack retinal vessels. Color Doppler Optical Coherence Tomography has the potential to obtain spatially resolved measurements of blood velocity in the eye’s choroidal circulation, but satisfactory velocity resolution in animal models remains elusive because of inherently low contrast across the retinal and choroid. Here we used a class of high-aspect ratio, 70 nm long. Highly scattering silver nanocrystals, as contrast agents in micro-etched flow phantoms with geometries based on the choroidal circulations mammalian eyes. OCT scans were taken before and after injection of the nanospheres. Results: Tomographic velocity images of the flow phantoms show marked enhancement when the flow contains the nano-particles. Contrast in relation to non-enhanced blood and surrounding material may be increased and relative mean velocity measurements show improved contrast and increased signal-to-noise ratio in the Doppler measurement. Conclusion: Nanoparicles, if they can be tolerated in vivo, have the potential to be used as contrast agents in Doppler OCT measurements in the eye.
This PDF is available to Subscribers Only