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DL Kent, C Sheridan, H Tomkinson, J Pearlman, P Hiscott; The Role of Peanut Lectin in Human Retinal Pigment Epithelial Cell De-Differentiation and Proliferation . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4514.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:De-differentiation of the retinal pigment epithelium (RPE) plays a pivotal role in such diverse retinal conditions as proliferative vitreoretinopathy in retinal detachment and disciform scaring in choroidal neovascularization. We investigated the role of glycoconjugate expression in human RPE de-differentiation using peanut lectin (PNL) from Arachis hypogaea. Methods:PNL immunohistochemistry of whole eyes, epiretinal membranes (ERMs) and HRPE cell cultures was performed. A Flouorescein isothiocyanate (FITC)-labelled PNL time course assay of binding to RPE cells was also performed. The effect of PNL on RPE proliferation was studied using a bromodeoxyuridine (BRDU) assay together with cytotoxicity analysis. Results:PNL binds RPE in ERMs but not the normal RPE monolayer. In vitro studies demonstrated that PNL bound de-differentiated RPE in a time-dependent manner and this binding caused a dose-dependent inhibition of RPE proliferation that was non-cytotoxic. Conclusion:PNL may have a role in the modification of anomalous RPE behaviour in a variety of retinal diseases characterized by aberrant wound healing.
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