Purchase this article with an account.
J Jumper, JE Kalns, K Batey, R Lane, J Reed; The 3-Nitrotyrosine Marker of Apoptosis Shows Time-Dependent and Tissue-Specific Variation in a Porcine Retinal Laser Injury Model . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4528.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Retinal laser lesions are known to enlarge with time, possibly due, in part, to apoptosis. We studied the changes in the production of the apoptosis marker, 3-nitrotyrosine (3-NT), with respect to time from laser application and distance from the epicenter of laser injury. Methods: 4-7 week old Sus scrofa pigs received retinal diode laser burns at varying time intervals between 0 and 72 hours using a precise time-to-fixation technique. 3-NT staining was performed and imaged using confocal microscopy. Results: We measured red 3-NT associated fluorescence in 4 retinal regions. Mean signal per region was recorded for each slide that was exposed to 3-NT antibody and a separate slide, exposed to an irrelevant antibody was used as a negative control. The signal attributable to non-specific binding as determined in the negative control was subtracted from the comparable 3-NT slide. R1 - Inner nuclear layer, R2 - Outer nuclear layer, R3 - Photoreceptor outer segments, R4 - Choriocapillaris. View OriginalDownload SlideView OriginalDownload Slide Conclusion: 3-NT production occurs mainly in the outer retina and varies depending on the age of the retinal injury as well as the distance from the epicenter of the injury.
This PDF is available to Subscribers Only