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R Kutty, RN Fariss, S Chen, W Samuel, T Duncan, CC Bridges, A El-Sherbeny, SB Smith, B Wiggert; Analysis Of The Expression Of NORPEG Protein In Retinal Pigment Epithelium And Other Ocular Tissues . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4551.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: NORPEG, a novel gene that we characterized from human retinal pigment epithelial (RPE) cells, encodes a putative 980 amino acid residue cytoskeletal protein containing six ankyrin repeats and a long coiled-coil domain (Kutty et al., J. Biol. Chem. 276: 2831, 2001). The expression of NORPEG protein in retinal pigment epithelium and other ocular tissues was analyzed in order to gain an insight into its functional role. Methods: Antibodies were raised in rabbits against synthetic peptides designed from the amino acid sequence of NORPEG protein. The antibody preparations were characterized using Western immunoblot analysis and then employed for immunohistochemical analysis of mouse and monkey eye sections. Results: In mouse eye, intense NORPEG immunoreactivity was detected in RPE, ganglion cell layer, Müller cell fibres, lens epithelium, corneal epithelium and ciliary body. Immunostaining of monkey retina also showed NORPEG expression in RPE, ganglion cell layer and Müller cell fibres. Conclusion: NORPEG protein is expressed in several ocular tissues, especially those containing epithelial cell types. The immunolocalization and structural characteristics of NORPEG suggest that this protein may play an important role in cell structure and function in several ocular tissues.
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