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JD Simon, NM Haralampus-Grynaviski, LE Lamb, M Rozanowska, T Sarna, JM Burke; Examination Of Individual RPE Lipofuscin Granules As A Function Of Donor Age Using Optical And Spatial Microscopy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4555.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine spectral and spatial properties of lipofuscin granules extracted from RPE cells of individual human donor eyes of different ages. Methods: Lipofuscin granules were isolated from the RPE cells using published procedures. The individual granules were characterized by atomic force and spectrally-resolved confocal fluorescence microscopies. Results: A broad distribution in the emission maximum of the spectra from individual lipofuscin granules isolated from single donors is observed. The average spectrum is in good agreement with previously published results from pooled samples. The distribution of emission maxima broadens with increased age of the donor eye. Conclusion: The distribution of fluorophores in lipofuscin varies among individual granules from single donors and differs for different aged eyes.
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