December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Trophism of AAV2 and AAV5 Vectors in the Non-human Primate Retina
Author Affiliations & Notes
  • AJ Lotery
    Ophthalmology
    University of Iowa Hospitals & Clinics Iowa City IA
  • TA Derksen
    Internal Medicine Neurology & Physiology & Biophysics
    University of Iowa Hospitals & Clinics Iowa City IA
  • SR Russell
    Ophthalmology
    University of Iowa Hospitals & Clinics Iowa City IA
  • RF Mullins
    Ophthalmology
    University of Iowa Hospitals & Clinics Iowa City IA
  • GS Yang
    Internal Medicine Neurology & Physiology & Biophysics
    University of Iowa Hospitals & Clinics Iowa City IA
  • KK Kopp
    Ophthalmology
    University of Iowa Hospitals & Clinics Iowa City IA
  • CG Eastman
    Ophthalmology
    University of Iowa Hospitals & Clinics Iowa City IA
  • EM Stone
    Ophthalmology
    University of Iowa Hospitals & Clinics Iowa City IA
  • BL Davidson
    Internal Medicine Neurology & Physiology & Biophysics
    University of Iowa Hospitals & Clinics Iowa City IA
  • Footnotes
    Commercial Relationships   A.J. Lotery, None; T.A. Derksen, None; S.R. Russell, None; R.F. Mullins, None; G.S. Yang, None; K.K. Kopp, None; C.G. Eastman, None; E.M. Stone, None; B.L. Davidson, None. Grant Identification: RPB Career Development Award (AL), University of Iowa Gene Therapy Center grant NIH/NIDDK, NIH P30D
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4609. doi:
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    • Get Citation

      AJ Lotery, TA Derksen, SR Russell, RF Mullins, GS Yang, KK Kopp, CG Eastman, EM Stone, BL Davidson; Trophism of AAV2 and AAV5 Vectors in the Non-human Primate Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4609.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate gene transfer with adeno-associated (AAV) viral vectors, serotypes 2 and 5 in the non-human primate retina Methods: Three rhesus monkeys underwent pars plana vitrectomy. A modified Hamilton syringe with a 41 gauge soft tipped cannula was passed through the vitreous cavity and multiple sub-retinal injections of a vector solution were placed in the sub-retinal space. The vector solution consisted of an equal mixture of CMVAAV2DSRed and CMVAAV5EGFP. Following surgery the animals were examined by ophthalmoscopy and electrophysiology before being sacrificed at 5, 8 or 9 months after surgery. The retinas were evaluated at the final endpoint by routine histology and immunohistochemistry. Results: One animal exhibited self-limited choroidal effusions post-operatively. There were no other surgical complications. All three animals exhibited normal electrophysiology following surgery. In vivo green fluorescence was visible one month following surgery in the two animals that did not develop choroidal effusions. One of these two animals was sacrificed 5 months after surgery and exhibited robust green fluorescence microscopically. This fluorescence was confirmed as originating from rod photoreceptors by immunohistochemistry. When the second animal was sacrificed at 9 months, green fluorescence was also detected but at a lower level. Conclusion: AAV5 appears to exhibit greater trophism for rod photoreceptors than AAV2 in the non-human primate retina. Transduction was most effective in the absence of post-operative effusion. With the conditions employed in this experiment, expression of the transgene appears to decrease with time.

Keywords: 419 gene transfer/gene therapy • 554 retina 
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